Analysis Note

ControlMEF1 cell lysate

Application

Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.

Research CategoryEpigenetics & Nuclear Function

Chromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.

Immunogen

MBP-tagged recombinant protein corres-ponding to full length human BMI1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Anti-BMI1, clone AF27

Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated BMI1 from 100 µg of MEF1 cell lysate.

Specificity

This antibody recognizes BMI1.

Storage and Stability

Stable at 2-8°C for 1 year from date of receipt.

Target description

~35 kDa